Learning American culture in Japan

Well I was really tired, but Ueno-san just popped in the door to lend me a Queen's c.d. HA! He asked if I listened to them, and I actually didn't know who they were. I'm listening to Radio Ga-Ga right now and I love it. I have heard some of these songs I guess, but I didn't know who was singing them

I drove myself crazy today with staining, trying to figure out why there was so much variability. I think I have it narrowed down to larval age and stretch, and have just finally had a good series of fibers. With staining it is very important not to pick guys that are too old as their boutons can be too enveloped by muscle to get good staining. I think the idea to destain with 60mM KCl confused the issue as well as that doesn't seem to be powerful enough to unload all of the boutons equally. We're trying to magnify a difference between Lat A and control unloading, but I'm excited about throwing it into an ephys experiment tomorrow to see what happens. I want to get at that 10 Hz pool, whatever it is.

Last night I confirmed to myself that I have no sense of direction. Sometimes in CO I fool myself into thinking I'm good (cheating with mountains). I was pedaling all over town last night looking for the 24-hr conveniance store. It's very close to my house with only two major turns, but I sampled all possibilities, in the rain, before finally getting there. Sigh.

I'm going to fall out of my chair despite the rock 'n roll, must head out.